PX'Therapeutics supports its customers from the early stages of their projects. From protein engineering to production of first batches for activity testing or proof-of-concept studies, we advise our customer based on our expertise and propose customised feasibility study programmes according to your specific needs.
We can assist you with:
Designing the candidate
Optimisation of challenging protein properties such as activity, affinity, stability and expression level.
Modification of antibody sequence to increase stability, limit aggregation or reduce immunogenicity.
Optimisation of gene sequence for expression in selected host, gene synthesis, mutagenesis and cloning.
Choosing the best expression strategy
Screening of multiple expression systems: bacteria, yeasts and mammalian cells.
For bacteria (E.coli): assessment of strains, vectors, expression location (periplasmic or cytoplasmic), medium.
For yeast: assessment of several strains of yeast, inducible or constitutive expression.
For mammalian cells: assessment of several cell lines, stable pool development using classical methods, transduction with lentiviral vectors (constitutive or inducible) for difficult-to-express or toxic proteins.
Setting up the production process
Set-up of culture and production protocols to maximise protein quality and yield
Design of optimal purification schemes using affinity, ion exchange, hydrophobic interaction, mixed-mode and size-exclusion chromatographies.
Specific expertise for proteins expressed in inclusion bodies in E. coli: advanced preparation of inclusion bodies and design of refolding experiments.